RT Journal Article
SR Electronic
T1 Modelling cell-cell interactions from spatial molecular data with spatial variance component analysis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 265256
DO 10.1101/265256
A1 Damien Arnol
A1 Denis Schapiro
A1 Bernd Bodenmiller
A1 Julio Saez-Rodriguez
A1 Oliver Stegle
YR 2018
UL http://biorxiv.org/content/early/2018/02/14/265256.abstract
AB Technological advances allow for assaying multiplexed spatially resolved RNA and protein expression profiling of individual cells, thereby capturing physiological tissue contexts of single cell variation. While methods for assaying spatial expression profiles are increasingly accessible, there is a lack of computational approaches that allow for studying the relevance of the spatial organization of tissues on cell-cell heterogeneity. Here, we present spatial variance component analysis (SVCA), a computational framework for the analysis of spatial molecular data. SVCA estimates signatures of spatial variance components, thereby quantifying the effect of cell-cell interactions, as well as environmental and intrinsic cell features on the expression levels of individual molecules. In application to a breast cancer Imaging Mass Cytometry dataset, our model yields robust spatial variance signatures, identifying cell-cell interactions as a major driver of expression heterogeneity. We also apply SVCA to high-dimensional imaging-derived RNA data, where we identify molecular pathways that are linked to cell-cell interactions.