RT Journal Article
SR Electronic
T1 CD8+ lymphocytes modulate Zika virus dynamics and tissue dissemination and orchestrate antiviral immunity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 475418
DO 10.1101/475418
A1 Blake Schouest
A1 Marissa Fahlberg
A1 Elizabeth A. Scheef
A1 Matthew J. Ward
A1 Kyra Headrick
A1 Dawn M. Szeltner
A1 Robert V. Blair
A1 Margaret H. Gilbert
A1 Lara A. Doyle-Meyers
A1 Victoria W. Danner
A1 Myrna C. Bonaldo
A1 Dawn M. Wesson
A1 Antonito T. Panganiban
A1 Nicholas J. Maness
YR 2019
UL http://biorxiv.org/content/early/2019/07/11/475418.abstract
AB CD8+ lymphocytes are critically important in the control of viral infections, but their roles in acute Zika virus (ZIKV) infection remain incompletely explored in a model sufficiently similar to humans immunologically. Here, we use CD8+ lymphocyte depletion to dissect acute immune responses in adult male rhesus and cynomolgus macaques infected with ZIKV. CD8 depletion delayed serum viremia and dysregulated patterns of innate immune cell homing and monocyte-driven transcriptional responses in the blood. CD8-depleted macaques also showed evidence of compensatory adaptive immune responses, with elevated Th1 activity and persistence of neutralizing antibodies beyond the clearance of serum viremia. The absence of CD8+ lymphocytes increased viral burdens in lymphatic tissues, semen, and cerebrospinal fluid, and neural lesions were also evident in both CD8-depleted rhesus macaques. Together, these data support a role for CD8+ lymphocytes in the control of ZIKV dissemination and in maintaining immune regulation during acute infection of nonhuman primates.