RT Journal Article
SR Electronic
T1 Analysis of mutations in West Australian populations of <em>Blumeria graminis</em> f. sp. <em>hordei CYP51</em> conferring resistance to DMI fungicides
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 696906
DO 10.1101/696906
A1 M. A. Tucker
A1 F. Lopez-Ruiz
A1 H. J. Cools
A1 J. G. L. Mullins
A1 K. Jayasena
A1 R. P. Oliver
YR 2019
UL http://biorxiv.org/content/early/2019/07/12/696906.abstract
AB Powdery mildew caused by Blumeria graminis f. sp. hordei (Bgh) is a constant threat to barley production but is generally well controlled through combinations of host genetics and fungicides. An epidemic of barley powdery mildew was observed from 2007 to 2013 in the West Australian wheatbelt (WA). We collected isolates, examined their sensitivity to demethylation inhibitor (DMI) fungicides and sequenced the Cyp51B target gene. Five amino acid substitutions were found of which four were novel. A clear association was established between combinations of mutations and altered levels of resistance to DMIs. The most resistant genotypes increased in prevalence from 0 in 2009 to 16% in 2010 and 90% in 2011. Yeast strains expressing the Bgh Cyp51 genotypes replicated the altered sensitivity to various DMIs and these results were confirmed by in silico protein docking studies.CypCyproconazoleDesthioDesthioconazoleEpoxiEpoxiconazoleFluquinFluquinconazoleFlutFlutriafolPropiPropiconazoleProthioProthioconazoleTebTebuconazoleTriadTriadimefon