PT  - JOURNAL ARTICLE
AU  - Tiansheng Li
AU  - Juan Ye
AU  - Lei Wang
AU  - Lin Zou
AU  - Yameng Guo
AU  - Linlin Hou
AU  - Danfeng Shen
AU  - Xiaohong Cai
AU  - Haobo Huang
AU  - Guiqin Sun
AU  - Li Chen
TI  - Bacterial fucosidase enables the production of Bombay red blood cells
AID  - 10.1101/695213
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 695213
4099  - http://biorxiv.org/content/early/2019/07/12/695213.short
4100  - http://biorxiv.org/content/early/2019/07/12/695213.full
AB  - We present a method to produce H antigen-deficient red blood cells (RBCs) for transfusion to individuals with anti-H antibodies. A fucosidase from bacteria was heterologously expressed efficiently in E. coli and has been demonstrated to completely remove H antigens on the surface of human RBCs in a facile conversion process. The approach we describe here holds promise for making H-deficient RBCs available for a rare population beyond ABO types.