PT  - JOURNAL ARTICLE
AU  - Carlos Bueno
AU  - Marta Martínez-Morga
AU  - Salvador Martínez
TI  - Non-proliferative adult neurogenesis in neural crest-derived stem cells isolated from human periodontal ligament
AID  - 10.1101/325613
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 325613
4099  - http://biorxiv.org/content/early/2019/07/13/325613.short
4100  - http://biorxiv.org/content/early/2019/07/13/325613.full
AB  - Understanding the sequence of events from undifferentiated stem cells to neuron is not only important for the basic knowledge of stem cell biology, but also for therapeutic applications. The aim of the present study is to evaluate the sequence of biological events occurring during neural differentiation of human periodontal ligament stem cells (hPDLSCs). Here, we show that hPDLSCs-derived neurons display a sequence of morphologic development highly similar to those reported before in primary neuronal cultures derived from rodent brains. We observed that cell proliferation is not present through neurogenesis from hPDLSCs. Futhermore, we may have discovered micronuclei movement and transient cell nuclei lobulation coincident to in vitro neurogenesis. Morphological analysis also reveals that neurogenic niches in the adult mouse brain contains cells with nuclear shapes highly similar to those observed during in vitro neurogenesis from hPDLSCs. Our results provide additional evidence that it is possible to differentiate hPDLSCs to neuron and suggest the possibility that the sequence of events from stem cell to neuron does not necessarily requires cell division from stem cell.