RT Journal Article
SR Electronic
T1 Non-proliferative adult neurogenesis in neural crest-derived stem cells isolated from human periodontal ligament
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 325613
DO 10.1101/325613
A1 Carlos Bueno
A1 Marta Martínez-Morga
A1 Salvador Martínez
YR 2019
UL http://biorxiv.org/content/early/2019/07/13/325613.abstract
AB Understanding the sequence of events from undifferentiated stem cells to neuron is not only important for the basic knowledge of stem cell biology, but also for therapeutic applications. The aim of the present study is to evaluate the sequence of biological events occurring during neural differentiation of human periodontal ligament stem cells (hPDLSCs). Here, we show that hPDLSCs-derived neurons display a sequence of morphologic development highly similar to those reported before in primary neuronal cultures derived from rodent brains. We observed that cell proliferation is not present through neurogenesis from hPDLSCs. Futhermore, we may have discovered micronuclei movement and transient cell nuclei lobulation coincident to in vitro neurogenesis. Morphological analysis also reveals that neurogenic niches in the adult mouse brain contains cells with nuclear shapes highly similar to those observed during in vitro neurogenesis from hPDLSCs. Our results provide additional evidence that it is possible to differentiate hPDLSCs to neuron and suggest the possibility that the sequence of events from stem cell to neuron does not necessarily requires cell division from stem cell.