RT Journal Article
SR Electronic
T1 Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 701359
DO 10.1101/701359
A1 Bruno Pok Man Ngou
A1 Hee-Kyung Ahn
A1 Pingtao Ding
A1 Amey Redkar
A1 Hannah Brown
A1 Yan Ma
A1 Mark Youles
A1 Laurence Tomlinson
A1 Jonathan DG Jones
YR 2019
UL http://biorxiv.org/content/early/2019/07/14/701359.abstract
AB Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis NLRs RRS1-R and RPS4, that confer recognition of bacterial effectors AvrRps4 and PopP2, are well studied, but how the RRS1/RPS4 complex activates early immediate downstream responses upon effector detection is still poorly understood. To study RRS1/RPS4 responses without the influence of cell-surface receptor immune pathways, we generated an Arabidopsis line with inducible expression of effector AvrRps4. Induction does not lead to hypersensitive cell death response (HR) but can induce electrolyte leakage, which often correlates with plant cell death. Activation of RRS1 and RPS4 without pathogens cannot activate mitogen-associated protein kinase cascades, but still activates upregulation of defense genes, and therefore resistance against bacteria.Highlight Inducible expression of AvrRps4 activates RRS1/RPS4-mediated effector-triggered immunity without the presence of pathogens, allowing us to characterise downstream immune responses triggered by TIR-NLRs without cell-surface receptor-mediated immunity.