RT Journal Article
SR Electronic
T1 Functional Annotation of Human Long Non-Coding RNAs via Molecular Phenotyping
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 700864
DO 10.1101/700864
A1 Jordan Ramilowski
A1 Chi Wai Yip
A1 Saumya Agrawal
A1 Jen-Chien Chang
A1 Yari Ciani
A1 Ivan V Kulakovskiy
A1 Mickael Mendez
A1 Jasmine Li Ching Ooi
A1 John F Ouyang
A1 Nick Parkinson
A1 Andreas Petri
A1 Leonie Roos
A1 Jessica Severin
A1 Kayoko Yasuzawa
A1 Imad Abugessaisa
A1 Altuna Akalin
A1 Ivan Antonov
A1 Erik Arner
A1 Alessandro Bonetti
A1 Hidemasa Bono
A1 Beatrice Borsari
A1 Frank Brombacher
A1 Carlo Vittorio Cannistraci
A1 Ryan Cardenas
A1 Melissa Cardon
A1 Howard Chang
A1 Josée Dostie
A1 Luca Ducoli
A1 Alexander Favorov
A1 Alexandre Fort
A1 Diego Garrido
A1 Noa Gil
A1 Juliette Gimenez
A1 Reto Guler
A1 Lusy Handoko
A1 Jayson Harshbarger
A1 Akira Hasegawa
A1 Yuki Hasegawa
A1 Kosuke Hashimoto
A1 Norihito Hayatsu
A1 Peter Heutink
A1 Tetsuro Hirose
A1 Eddie L Imada
A1 Masayoshi Itoh
A1 Bogumil Kaczkowski
A1 Aditi Kanhere
A1 Emily Kawabata
A1 Hideya Kawaji
A1 Tsugumi Kawashima
A1 Tom Kelly
A1 Miki Kojima
A1 Naoto Kondo
A1 Haruhiko Koseki
A1 Tsukasa Kouno
A1 Anton Kratz
A1 Mariola Kurowska-Stolarska
A1 Andrew Tae Jun Kwon
A1 Jeffrey Leek
A1 Andreas Lennartsson
A1 Marina Lizio
A1 Fernando Lopez
A1 Joachim Luginbühl
A1 Shiori Maeda
A1 Vsevolod Makeev
A1 Luigi Marchionni
A1 Yulia A Medvedeva
A1 Aki Minoda
A1 Ferenc Müller
A1 Manuel Munoz Aguirre
A1 Mitsuyoshi Murata
A1 Hiromi Nishiyori
A1 Kazuhiro Nitta
A1 Shuhei Noguchi
A1 Yukihiko Noro
A1 Ramil Nurtdinov
A1 Yasushi Okazaki
A1 Valerio Orlando
A1 Denis Paquette
A1 Callum Parr
A1 Owen JL Rackham
A1 Patrizia Rizzu
A1 Diego Fernando Sanchez
A1 Albin Sandelin
A1 Pillay Sanjana
A1 Colin AM Semple
A1 Harshita Sharma
A1 Youtaro Shibayama
A1 Divya Sivaraman
A1 Takahiro Suzuki
A1 Suzannah Szumowski
A1 Michihira Tagami
A1 Martin S Taylor
A1 Chikashi Terao
A1 Malte Thodberg
A1 Supat Thongjuea
A1 Vidisha Tripathi
A1 Igor Ulitsky
A1 Roberto Verardo
A1 Ilya Vorontsov
A1 Chinatsu Yamamoto
A1 Robert S Young
A1 J Kenneth Baillie
A1 Alistair RR Forrest
A1 Roderic Guigó
A1 Michael M Hoffman
A1 Chung Chau Hon
A1 Takeya Kasukawa
A1 Sakari Kauppinen
A1 Juha Kere
A1 Boris Lenhard
A1 Claudio Schneider
A1 Harukazu Suzuki
A1 Ken Yagi
A1 FANTOM consortium
A1 Michiel de Hoon
A1 Jay W Shin
A1 Piero Carninci
YR 2019
UL http://biorxiv.org/content/early/2019/07/14/700864.abstract
AB Long non-coding RNAs (lncRNAs) constitute the majority of transcripts in mammalian genomes and yet, their functions remain largely unknown. We systematically suppressed 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). The resulting transcriptomic profiles recapitulated the observed cellular phenotypes, yielding specific roles for over 40% of analyzed lncRNAs in regulating distinct biological pathways, transcriptional machinery, alternative promoter activity and architecture usage. Overall, combining cellular and molecular profiling provided a powerful approach to unravel the distinct functions of lncRNAs, which we highlight with specific functional roles for ZNF213-AS1 and lnc-KHDC3L-2.