RT Journal Article
SR Electronic
T1 TMT-Opsins differentially modulate medaka brain function in a context-dependent fashion
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 698480
DO 10.1101/698480
A1 Bruno M. Fontinha
A1 Theresa Zekoll
A1 Miguel Gallach
A1 Florian Reithofer
A1 Alison J. Barker
A1 Maximilian Hofbauer
A1 Ruth M. Fischer
A1 Arndt von Haeseler
A1 Herwig Baier
A1 Kristin Tessmar-Raible
YR 2019
UL http://biorxiv.org/content/early/2019/07/16/698480.abstract
AB Vertebrate behavior is strongly influenced by light. Photoreceptors, encoded by Opsins, are present inside the vertebrate brain and peripheral tissues. Their non-visual functions are largely enigmatic.We focus on tmt-opsin1b and 2, c-Opsins with ancestral-type sequence features, conserved across several vertebrate phyla and with partly similar expression. Their loss-of-function mutations differentially modulate medakafish behavior in a context-dependent manner. Specifically, differences in light conditions have differential effects depending on age and frequency of the light changes, part of which are mediated by TMT-Opsin1b acting outside the eyes, while the pre-pro-hormone sst1b is regulated by daylength via TMT-opsin1b in an eye-dependent manner. Analyses of tmt-opsin1b;tmt-opsin2 double mutants reveals partial complementation of single mutant behavioral and molecular phenotypes.Our work starts to disentangle the highly complex interactions of vertebrate non-visual Opsins, suggesting that tmt-opsin-expressing cells together with other Opsins provide detailed light information to the organism for behavioral fine-tuning.One Sentence Summary Two medakafish non-visual c-type Opsins interact non-additively, impacting the levels of the preprohormone sst1b, as well as the voltage-gated sodium channel subunit scn12aa and- at least in part independently of the eyes- the amount of larval day-time rest.