TY - JOUR T1 - Non-Redundant Roles in Sister Chromatid Cohesion of the DNA Helicase DDX11 and the SMC3 Acetyl Transferases ESCO1/2 JF - bioRxiv DO - 10.1101/704635 SP - 704635 AU - Atiq Faramarz AU - Jesper A. Balk AU - Anneke B. Oostra AU - Cherien A. Ghandour AU - Martin A. Rooimans AU - Rob M.F. Wolthuis AU - Job de Lange Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/07/16/704635.abstract N2 - In a process linked to DNA replication, duplicated chromosomes are co-entrapped into large, circular cohesin complexes and functional sister chromatid cohesion (SCC) is established by acetylation of the SMC3 cohesin subunit. Several rare human developmental syndromes are characterized by defective SCC. Roberts Syndrome (RBS) is caused by mutations in the SMC3 acetyl transferase ESCO2, whereas mutations in the DNA helicase DDX11 lead to Warsaw Breakage Syndrome (WABS). We found that WABS-derived cells predominantly rely on ESCO2, not ESCO1, for residual SCC, growth and survival. Reciprocally, RBS-derived cells depend on DDX11 to maintain low levels of SCC. Synthetic lethality between DDX11 and ESCO2 correlated with a prolonged delay in mitosis, and was rescued by knock down of the cohesion remover WAPL. Rescue experiments using mouse or human cDNAs revealed that DDX11, ESCO1 and ESCO2 act on differential aspects of DNA replication-coupled SCC establishment. Importantly, DDX11 is required for normal DNA replication fork speed without clearly affecting SMC3 acetylation. We propose that DDX11 and ESCO2 control spatially separated fractions of cohesin, with supportive roles for DDX11 in replication-associated cohesion establishment, and for ESCO2 in cohesin complexes located around centromeres, respectively. ER -