RT Journal Article
SR Electronic
T1 Cell types of the human retina and its organoids at single-cell resolution: developmental convergence, transcriptomic identity, and disease map
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 703348
DO 10.1101/703348
A1 Cameron S. Cowan
A1 Magdalena Renner
A1 Brigitte Gross-Scherf
A1 David Goldblum
A1 Martin Munz
A1 Jacek Krol
A1 Tamas Szikra
A1 Panagiotis Papasaikas
A1 Rachel Cuttat
A1 Annick Waldt
A1 Roland Diggelmann
A1 Claudia P. Patino-Alvarez
A1 Nadine Gerber-Hollbach
A1 Sven Schuierer
A1 Yanyan Hou
A1 Aldin Srdanovic
A1 Marton Balogh
A1 Riccardo Panero
A1 Pascal W. Hasler
A1 Akos Kusnyerik
A1 Arnold Szabo
A1 Michael B. Stadler
A1 Selim Orgül
A1 Andreas Hierlemann
A1 Hendrik P. N. Scholl
A1 Guglielmo Roma
A1 Florian Nigsch
A1 Botond Roska
YR 2019
UL http://biorxiv.org/content/early/2019/07/16/703348.abstract
AB How closely human organoids recapitulate cell-type diversity and cell-type maturation of their target organs is not well understood. We developed human retinal organoids with multiple nuclear and synaptic layers. We sequenced the RNA of 158,844 single cells from these organoids at six developmental time points and from the periphery, fovea, pigment epithelium and choroid of light-responsive adult human retinas, and performed histochemistry. Cell types in organoids matured in vitro to a stable ‘developed’ state at a rate similar to human retina development in vivo and the transcriptomes of organoid cell types converged towards the transcriptomes of adult peripheral retinal cell types. The expression of disease-associated genes was significantly cell-type specific in adult retina and cell-type specificity was retained in organoids. We implicate unexpected cell types in diseases such as macular degeneration. This resource identifies cellular targets for studying disease mechanisms in organoids and for targeted repair in adult human retinas.