RT Journal Article
SR Electronic
T1 Single-cell transcriptome profiling reveals dermal and epithelium cell fate decisions during embryonic hair follicle development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 704379
DO 10.1101/704379
A1 Wei Ge
A1 Shao-Jing Tan
A1 Shan-He Wang
A1 Lan Li
A1 Xiao-Feng Sun
A1 Wei Shen
A1 Xin Wang
YR 2019
UL http://biorxiv.org/content/early/2019/07/16/704379.abstract
AB Characterization of the morphological structure during hair follicle development has been well documented, while the current understanding of the molecular mechanisms involved in follicle development remain limited. Here, using unbiased single-cell RNA sequencing, we analyzed 15,086 single cell transcriptome profiles from E13.5 and E16.5 fetal mice, and newborn mouse (postnatal day 0, P0) dorsal skin cells. Based on t-distributed Stochastic Neighbor Embedding (tSNE) clustering, we identified 14 cell clusters from skin cells and delineated their cell identity gene expression profiles. Pseudotime ordering analysis successfully constructed epithelium/dermal cell lineage differentiation trajectory and revealed sequential activation of key regulons involved during cell fate decisions. Along with this, intercellular communication between different cell populations were inferred based on a priori knowledge of ligand-receptor pairs. Together, our findings here provide a molecular landscape during hair follicle epithelium/dermal cell lineage fate decisions, and more importantly, recapitulate sequential activation of core regulatory transcriptional factors for different cell populations during hair follicle morphogenesis.