TY - JOUR T1 - Single-Cell RNA-Seq Reveals Novel Mitochondria-related Musculoskeletal Cell Populations during Adult Axolotl Limb Regeneration Process JF - bioRxiv DO - 10.1101/704841 SP - 704841 AU - Tian Qin AU - Chun-mei Fan AU - Ting-zhang Wang AU - Long Yang AU - Wei-liang Shen AU - Heng Sun AU - Jun-xin Lin AU - Magali Cucchiarini AU - Nicholas D. Clement AU - Christopher E. Mason AU - Varitsara Bunpetch AU - Norimasa Nakamura AU - Rameah Bhonde AU - Nicholas D. Clement AU - Zi Yin AU - Xiao Chen Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/07/16/704841.abstract N2 - While the capacity to regenerate tissues or limbs is limited in mammals including humans, unlike us, axolotls are able to regrow entire limbs and major organs. The wound blastema have been extensively studied in limb regeneration. However, due to the inadequate characterization and coordination of cell subpopulations involved in the regeneration process, it hinders the discovery of the key clue for human limb regeneration. In this study, we applied unbiased large-scale single-cell RNA sequencing to classify cells throughout the adult axolotl limb regeneration process. We computationally identified 7 clusters in regenerating limbs, including the novel regeneration-specific mitochondria-related cluster supporting regeneration through energy providing and the COL2+ cluster contributing to regeneration through cell-cell interactions signals. We also discovered the dedifferentiation and re-differentiation of the COL1+/COL2+ cellular subpopulation and uncovered a COL2-mitochondria sub-cluster supporting the musculoskeletal system regeneration. On the basis of these findings, we reconstructed the dynamic single-cell transcriptome atlas of adult axolotl limb regenerative process, and identified the novel regenerative mitochondria-related musculoskeletal populations, which yielded deeper insights into the crucial interactions between cell clusters within the regenerative microenvironment. ER -