RT Journal Article
SR Electronic
T1 Identification of a major QTL and associated marker for high arabinoxylan fibre in white wheat flour
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 705343
DO 10.1101/705343
A1 Alison Lovegrove
A1 Luzie U. Wingen
A1 Amy Plummer
A1 Abigail Wood
A1 Diana Passmore
A1 Ondrej Kosik
A1 Jackie Freeman
A1 Rowan A.C. Mitchell
A1 Mehmet Ulker
A1 Karolina Tremmel-Bede
A1 Marianna Rakszegi
A1 Zoltán Bedő
A1 Marie-Reine Petterant
A1 Gilles Charmet
A1 Michelle Leverington Waite
A1 Simon Orford
A1 Amanda Burridge
A1 Till Pellny
A1 Peter R Shewry
A1 Simon Griffiths
YR 2019
UL http://biorxiv.org/content/early/2019/07/17/705343.abstract
AB Dietary fibre (DF) has multiple health benefits, and wheat products are major sources of DF for human health. However, DF is depleted in white flour, which is most widely consumed, compared to wholegrain. The major type of DF in white wheat flour is the cell wall polysaccharide arabinoxylan (AX). Previous studies have identified the Chinese wheat cultivar Yumai 34 as having unusually high contents of AX in both water-soluble and insoluble forms. We have therefore used populations generated from crosses between Yumai 34 and four other wheat cultivars, three with average contents of AX (Ukrainka, Altigo and Claire) and one also having unusually high AX (Valoris), in order to map QTLs for soluble AX (determined as relative viscosity) of aqueous extracts of wholemeal flours) and total AX (determined by enzyme fingerprinting of white flour). A number of QTL were mapped, but most were only detected in one or two crosses. However, all four crosses showed strong QTLs for high RV/total AX on chromosome 1B, with Yumai 34 being the increasing parent, and a KASP marker for the high AX Yumai 34 allele was validated by analysis of high AX lines derived from Yumai 34 but selected by biochemical analysis. A QTL for RV was mapped on chromosome 6B in Yumai 34 × Valoris, with Valoris being the increasing allele, which was consistent with the observation of transgressive segregation for this trait. The data indicate that breeding can be used to develop wheat with high AX fibre in white flour.