TY - JOUR T1 - The <em>C. elegans</em> 3’UTRome V2: an updated genomic resource to study 3’UTR biology JF - bioRxiv DO - 10.1101/704098 SP - 704098 AU - HS Steber AU - C Gallante AU - S O’Brien AU - P.-L Chiu AU - M Mangone Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/07/18/704098.abstract N2 - 3’Untranslated Regions (3’UTRs) of mRNAs emerged as central regulators of cellular function as they contain important but poorly-characterized cis-regulatory elements targeted by a multitude of regulatory factors. The soil nematode C. elegans is an ideal model to study these interactions since it possesses a well-defined 3’UTRome. In order to improve its annotation, we have used a genomics approach to download raw transcriptome data for ~1,500 transcriptome datasets corresponding to the entire collection of C. elegans trancriptomes from 2015 to 2018 from the Sequence Read Archive at the NCBI. We then extracted and mapped high-quality 3’UTR data at ultra-deep coverage. Here we describe and release to the Community the updated version of the worm 3’UTRome, which we named 3’UTRome v2. This resource contains high-quality 3’UTR data mapped at single base ultra-resolution for 23,159 3’UTR isoforms variants corresponding to 14,808 protein-coding genes and is updated to the latest release of WormBase. We used this dataset to study and probe principles of RNA cleavage and polyadenylation in C. elegans. The worm 3’UTRome v2 represents the most comprehensive and high-resolution 3’UTR dataset available in C. elegans, and provides a novel resource to investigate the mRNA cleavage and polyadenylation reaction, 3’UTR biology and miRNA targeting in a living organism. ER -