TY - JOUR T1 - Highly efficient CRISPR/Cas9-mediated tissue specific mutagenesis in <em>Drosophila</em> JF - bioRxiv DO - 10.1101/268482 SP - 268482 AU - Amy R. Poe AU - Bei Wang AU - Maria L. Sapar AU - Hui Ji AU - Kailyn Li AU - Tireniolu Onabajo AU - Rushaniya Fazliyeva AU - Mary Gibbs AU - Yue Qiu AU - Yuzhao Hu AU - Chun Han Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/02/20/268482.abstract N2 - Tissue-specific loss-of-function (LOF) analysis is an essential approach for characterizing gene function. Here we describe an efficient CRISPR-mediated tissue-restricted mutagenesis (CRISPR-TRiM) method for ablating gene function in Drosophila. This binary system consists of a tissue-specific Cas9 and a ubiquitously expressed multi-guide RNA (gRNA) transgene. To facilitate the construction of these components, we developed convenient tools for generating and evaluating enhancer-driven Cas9 lines, identified a multi-gRNA design that is highly efficient in mutagenizing somatic cells, and established an assay for testing the efficiency of multi-gRNAs in creating double-stranded breaks. We found that excision of genomic DNA induced by two gRNAs is infrequent in somatic cells, while indels more reliably cause tissue-specific LOF. Furthermore, we show that enhancer-driven Cas9 is less cytotoxic yet results in more complete gene removal than Gal4-driven Cas9 in larval neurons. Finally, we demonstrate that CRISPR-TRiM efficiently unmasks redundant gene functions in neuronal morphogenesis. Importantly, two Cas9 transgenes that turn on with different timings in the neuronal lineage revealed the extent to which gene products persist in cells after tissue-specific gene knockout. These CRISRPR tools can be applied to analyze tissue-specific gene function in many biological processes. ER -