RT Journal Article
SR Electronic
T1 Unveiling The Worldwide UV-Metaresistome: Metagenomic Analysis of Microbiomes Exposed to Different UV-B Regimes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 701565
DO 10.1101/701565
A1 Daniel G. Alonso-Reyes
A1 Maria Eugenia Farias
A1 Virginia Helena Albarracín
YR 2019
UL http://biorxiv.org/content/early/2019/07/18/701565.abstract
AB During evolution, microorganisms exposed to high UV doses have developed a complex battery of physiological and molecular mechanisms to cope with UV stress and damage recently called as the UV-resistome. As a precedent, the UV-resistome was analyzed at the genomic and proteomic level in poly-extremophiles bacteria isolated from High-Altitude Andean Lakes. In this work, we go further by exploring the impact of UV-B radiation on microbiomes in different geographic regions across the globe. The abundance of photoprotection and DNA repair pathways in each microbiome was use to configure the world-wide UV-metaresistome. Metagenomics combined with georeferenced climate data indicated that the higher the UV-B dose suffered by the microbiome the higher the abundance and diversity of the UV-metaresistome genes. In contrast, a substantial depletion on microbial diversity was observed in higher irradiated environments. A positive correlation between CPF abundance and radiation intensity/photoperiod was detected. CPF genes were investigated in the global dataset, being present in most abundant organisms of communities that are facing significant exposure to sunlight. Three extra clades not identified so far and a widespread occurrence of cryptochromes-related genes were reported.