RT Journal Article
SR Electronic
T1 Neuronal receptors display cytoskeleton-independent directed motion on the plasma membrane
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 269381
DO 10.1101/269381
A1 R. D. Taylor
A1 M. Heine
A1 N. J. Emptage
A1 L. C. Andreae
YR 2018
UL http://biorxiv.org/content/early/2018/02/21/269381.abstract
AB Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe which specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity.