RT Journal Article
SR Electronic
T1 Characterization of directed differentiation by high-throughput single-cell RNA-Seq
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 003236
DO 10.1101/003236
A1 Magali Soumillon
A1 Davide Cacchiarelli
A1 Stefan Semrau
A1 Alexander van Oudenaarden
A1 Tarjei S. Mikkelsen
YR 2014
UL http://biorxiv.org/content/early/2014/03/05/003236.abstract
AB Directed differentiation of cells in vitro is a powerful approach for dissection of developmental pathways, disease modeling and regenerative medicine, but analysis of such systems is complicated by heterogeneous and asynchronous cellular responses to differentiation-inducing stimuli. To enable deep characterization of heterogeneous cell populations, we developed an efficient digital gene expression profiling protocol that enables surveying of mRNA in thousands of single cells at a time. We then applied this protocol to profile 12,832 cells collected at multiple time points during directed adipogenic differentiation of human adipose-derived stem/stromal cells in vitro. The resulting data reveal the major axes of cell-to-cell variation within and between time points, and an inverse relationship between inflammatory gene expression and lipid accumulation across cells from a single donor.