RT Journal Article
SR Electronic
T1 Mid-infrared optoacoustic microscopy with label-free chemical contrast in living cells and tissues
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 270082
DO 10.1101/270082
A1 Miguel A. Pleitez
A1 Asrar Ali Khan
A1 Josefine Reber
A1 Andriy Chmyrov
A1 Markus R. Seeger
A1 Stephan Herzig
A1 Marcel Scheideler
A1 Vasilis Ntziachristos
YR 2018
UL http://biorxiv.org/content/early/2018/02/22/270082.abstract
AB We developed mid-infrared optoacoustic microscopy (MiROM), a bond-selective imaging modality that overcomes water/tissue opacity and depth limitations of mid-infrared sensing allowing uncompromised live-cell/thick-tissue mid-infrared microscopy with up to three orders of magnitudehigher sensitivity than other vibrational imaging modalities; such as Raman. We showcase the functional label-free biomolecular imaging capabilities of MiROM by monitoring the spatiotemporal dynamics of lipids and proteins during lipolysis in living adipocytes. Since MiROM, contrary to Ramanmodalities, is not only able to detect lipids and proteins, but also important metabolites such as glucose without the need of labels, here we discuss how MiROM yields novel functional label-free abilities for a broader range of analytical studies in living cells and tissues.