RT Journal Article
SR Electronic
T1 Quantitative solid-phase assay to measure deoxynucleoside triphosphate pools
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 270454
DO 10.1101/270454
A1 Juan Cruz Landoni
A1 Liya Wang
A1 Anu Suomalainen
YR 2018
UL http://biorxiv.org/content/early/2018/02/23/270454.abstract
AB Deoxyribonucleoside triphosphates (dNTPs) are the reduced nucleotides used as the building blocks and energy source for DNA replication and maintenance in all living systems. They are present in highly regulated amounts and ratios in the cell, and their balance has been implicated in the most important cell processes, from determining the fidelity of DNA replication to affecting cell fate. Furthermore, many cancer drugs target biosynthetic enzymes in dNTP metabolism, and mutations in genes directly or indirectly affecting these pathways are the cause of devastating diseases. The accurate and systematic measurement of these pools is key to understand the mechanisms behind these diseases and their treatment. We present a new method for measuring dNTP pools from biological samples, utilising the current state-of-the-art polymerase method, modified to a solid-phase setting and optimised for larger scale measurements.