TY - JOUR T1 - CBP80/20-dependent translation initiation factor (CTIF) inhibits HIV-1 Gag synthesis by targeting the function of the viral protein Rev JF - bioRxiv DO - 10.1101/710137 SP - 710137 AU - Francisco García-de-Gracia AU - Daniela Toro-Ascuy AU - Sebastián Riquelme-Barrios AU - Camila Pereira-Montecinos AU - Bárbara Rojas-Araya AU - Aracelly Gaete-Argel AU - Mónica L. Acevedo AU - Jonás Chnaiderman AU - Fernando Valiente-Echeverría AU - Ricardo Soto-Rifo Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/07/22/710137.abstract N2 - Translation initiation of the human immunodeficiency virus type-1 (HIV-1) unspliced mRNA has been shown to occur through cap-dependent and IRES-driven mechanisms. Previous studies suggested that the nuclear cap-binding complex (CBC) rather than eIF4E drives cap-dependent translation of the unspliced mRNA and we have recently reported that the CBC subunit CBP80 supports the function of the viral protein Rev during nuclear export and translation of this viral transcript. Ribosome recruitment during CBC-dependent translation of cellular mRNAs relies on the activity CBP80/20 translation initiation factor (CTIF), which bridges CBP80 and the 40S ribosomal subunit through interactions with eIF3g. Here, we report that CTIF restricts HIV-1 replication by interfering with Gag synthesis from the unspliced mRNA. Our results indicate that CTIF associates with Rev through its N-terminal domain and is recruited onto the unspliced mRNA ribonucleoprotein complex in order to block translation. We also demonstrate that CTIF induces the cytoplasmic accumulation of Rev impeding the association of the viral protein with CBP80. We finally show that CTIF restricts HIV-2 but not MLV Gag synthesis indicating an inhibitory mechanism conserved in Rev-expressing human lentiviruses. ER -