RT Journal Article
SR Electronic
T1 Actin modulates shape and mechanics of tubular membranes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 712505
DO 10.1101/712505
A1 A. Allard
A1 M. Bouzid
A1 T. Betz
A1 C. Simon
A1 M. Abou-Ghali
A1 J. Lemière
A1 F. Valentino
A1 J. Manzi
A1 F. Brochard-Wyart
A1 K. Guevorkian
A1 J. Plastino
A1 M. Lenz
A1 C. Campillo
A1 C. Sykes
YR 2019
UL http://biorxiv.org/content/early/2019/07/23/712505.abstract
AB The actin cytoskeleton shapes cells and also organizes internal membranous compartments. In particular, it interacts with membranes in intracellular transport of material in mammalian cells, yeast or plant cells. Tubular membrane intermediates, pulled along microtubule tracks, are involved during these processes, and destabilize into vesicles. While the role of actin in this destabilization process is still debated, literature also provide examples of membranous structures stabilization by actin. To directly address this apparent contradiction, we mimic the geometry of tubular intermediates with preformed membrane tubes. The growth of an actin sleeve at the tube surface is monitored spatio-temporally. Depending on network cohesiveness, actin is able to stabilize, or maintain membrane tubes under pulling. Indeed, on a single tube, thicker portions correlate with the presence of actin. Such structures relax over several minutes, and may provide enough time and curvature geometries for other proteins to act on tube stability.