RT Journal Article
SR Electronic
T1 SMAD3 Determines Conventional versus Plasmacytoid Dendritic Cell Fates
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 715060
DO 10.1101/715060
A1 Jeong-Hwan Yoon
A1 Eunjin Bae
A1 Katsuko Sudo
A1 Jin Soo Han
A1 Seok Hee Park
A1 Susumu Nakae
A1 Tadashi Yamashita
A1 In-Kyu Lee
A1 Ji Hyeon Ju
A1 Isao Matsumoto
A1 Takayuki Sumida
A1 Masahiko Kuroda
A1 Keiji Miyazawa
A1 Mitsuyasu Kato
A1 Mizuko Mamura
YR 2019
UL http://biorxiv.org/content/early/2019/07/25/715060.abstract
AB Transforming growth factor (TGF)-β plays crucial roles in differentiation of dendritic cells (DC). However, molecular mechanisms how TGF-β regulates DC differentiation remain largely unknown. Here, we show that selective repression of one of the TGF-β receptor-regulated SMADs (R-SMADs), SMAD3 directs conventional DC (cDC) differentiation, whereas maintenance of SMAD3 is indispensable for plasmacytoid DC (pDC) differentiation. Expression of SMAD3 was specifically downregulated in CD115+ common DC progenitor (CDP), pre-cDCs and cDCs. SMAD3 deficient mice showed a significant reduction in pre-pDCs and pDCs with increased CDP, pre-cDCs and cDCs. SMAD3 upregulated the pDC-related genes: SPI-B, E2-2 and IKAROS, while it repressed FLT3 and the cDC-related genes: IRF4 and ID2. STAT3 and a SMAD transcriptional co-repressor, c-SKI repressed SMAD3 for cDC differentiation, whereas canonical SMAD-mediated TGF-β signalling maintained SMAD3 for pDC differentiation. Thus, SMAD3 is the pivotal determinant to bifurcate cDC and pDC differentiation in the steady-state condition.