PT  - JOURNAL ARTICLE
AU  - Yang, Bin
AU  - Wang, Yina
AU  - Feng, Siyu
AU  - Pessino, Veronica
AU  - Stuurman, Nico
AU  - Huang, Bo
TI  - High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy
AID  - 10.1101/273359
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 273359
4099  - http://biorxiv.org/content/early/2018/02/28/273359.short
4100  - http://biorxiv.org/content/early/2018/02/28/273359.full
AB  - Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for long-term volumetric imaging of living organisms. In order to achieve high-resolution imaging in common biological sample holders, we designed a high numerical aperture (NA) epi-illumination SPIM (eSPIM) system, which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. This system has an effective detection NA of > 1.06. We demonstrated multicolor and fast volumetric imaging of live cells and single-molecule super-resolution microscopy using our system.