RT Journal Article
SR Electronic
T1 High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 273359
DO 10.1101/273359
A1 Yang, Bin
A1 Wang, Yina
A1 Feng, Siyu
A1 Pessino, Veronica
A1 Stuurman, Nico
A1 Huang, Bo
YR 2018
UL http://biorxiv.org/content/early/2018/02/28/273359.abstract
AB Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for long-term volumetric imaging of living organisms. In order to achieve high-resolution imaging in common biological sample holders, we designed a high numerical aperture (NA) epi-illumination SPIM (eSPIM) system, which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. This system has an effective detection NA of > 1.06. We demonstrated multicolor and fast volumetric imaging of live cells and single-molecule super-resolution microscopy using our system.