RT Journal Article
SR Electronic
T1 Mass Spectrometry-based Plasma Proteomics: Considerations from Sample Collection to Achieving Translatable Data
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 716563
DO 10.1101/716563
A1 Vera Ignjatovic
A1 Philipp E Geyer
A1 Krishnan K Palaniappan
A1 Jessica E Chaaban
A1 Gilbert S Omenn
A1 Mark S Baker
A1 Eric W Deutsch
A1 Jochen M Schwenk
YR 2019
UL http://biorxiv.org/content/early/2019/07/26/716563.abstract
AB The proteomic analysis of human blood and blood-derived products, particularly plasma, offers an attractive avenue to translate research from the laboratory into the clinic. However, due to its particular protein composition, performing proteomics assays with plasma is challenging. Plasma proteomics has though regained interest due to recent technological advances, but challenges imposed by both the complications inherent to studying human biology (e.g. sample variability) and the limitations in available technologies and methods remain. As part of the Human Plasma Proteome Project (HPPP), we bring together key aspects of the plasma proteomics pipeline. We provide considerations and recommendations concerning the study design, plasma collection and quality metrics, plasma processing workflows, data acquisition by mass spectrometry (MS), data processing and bioinformatic analysis. With exciting opportunities in studying human health and disease via the plasma proteomics pipeline, a more informed analysis of plasma will accelerate interest and possibilities for the incorporation of proteomics-scaled assays into clinical practice.