PT  - JOURNAL ARTICLE
AU  - Dillon C. Muth
AU  - Bonita H. Powell
AU  - Zezhou Zhao
AU  - Kenneth W. Witwer
TI  - miRNAs in platelet-poor blood plasma and purified RNA are highly stable: a confirmatory study
AID  - 10.1101/273797
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 273797
4099  - http://biorxiv.org/content/early/2018/03/01/273797.short
4100  - http://biorxiv.org/content/early/2018/03/01/273797.full
AB  - The relative stability of microRNAs (miRNAs) as compared with other RNA molecules has been confirmed in many contexts. When bound to Argonaute (AGO) proteins, miRNAs are protected from degradation, even when released into the extracellular space in ribonucleoprotein complexes, and with or without the protection of membranes in extracellular vesicles (EVs). Purified miRNAs also appear to present less of a target for degradation than other RNAs. Although miRNAs are by no means immune to degradation, biological samples subjected to prolonged incubation at room temperature, multiple freeze/thaws, or collection in the presence of inhibitors like heparin, can typically be remediated or used directly for miRNA measurements. Here, we provide additional confirmation of early, well validated findings on miRNA stability and detectability. Our data also suggest that inadequate depletion of platelets from plasma may explain the occasional report that freeze-thaw cycles can adversely affect plasma miRNA levels. Overall, the repeated observation of miRNA stability is again confirmed.