RT Journal Article
SR Electronic
T1 A 6-nm ultra-photostable DNA Fluorocube for fluorescence imaging
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 716787
DO 10.1101/716787
A1 Stefan Niekamp
A1 Nico Stuurman
A1 Ronald D. Vale
YR 2019
UL http://biorxiv.org/content/early/2019/07/27/716787.abstract
AB Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA origami-based “Fluorocubes” that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a 50-fold higher photobleaching lifetime and emit 40-fold more photons than single organic dyes. We demonstrate that DNA Fluorocubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.