PT  - JOURNAL ARTICLE
AU  - Norris, Alexis L.
AU  - Lee, Stella S.
AU  - Greenlees, Kevin J.
AU  - Tadesse, Daniel A.
AU  - Miller, Mayumi F.
AU  - Lombardi, Heather
TI  - Template plasmid integration in germline genome-edited cattle
AID  - 10.1101/715482
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 715482
4099  - http://biorxiv.org/content/early/2019/07/28/715482.short
4100  - http://biorxiv.org/content/early/2019/07/28/715482.full
AB  - We analyzed publicly available whole genome sequencing data from cattle which were germline genome-edited to introduce polledness. Our analysis discovered the unintended heterozygous integration of the plasmid and a second copy of the repair template sequence, at the target site. Our finding underscores the importance of employing screening methods suited to reliably detect the unintended integration of plasmids and multiple template copies.