RT Journal Article
SR Electronic
T1 Template plasmid integration in germline genome-edited cattle
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 715482
DO 10.1101/715482
A1 Alexis L. Norris
A1 Stella S. Lee
A1 Kevin J. Greenlees
A1 Daniel A. Tadesse
A1 Mayumi F. Miller
A1 Heather Lombardi
YR 2019
UL http://biorxiv.org/content/early/2019/07/28/715482.abstract
AB We analyzed publicly available whole genome sequencing data from cattle which were germline genome-edited to introduce polledness. Our analysis discovered the unintended heterozygous integration of the plasmid and a second copy of the repair template sequence, at the target site. Our finding underscores the importance of employing screening methods suited to reliably detect the unintended integration of plasmids and multiple template copies.