@article {Wang274456, author = {Yu Wang and Wenxin Xie and Richie E. Kohman and George M. Church}, title = {Multiplexed imaging using same species primary antibodies with signal amplification}, elocation-id = {274456}, year = {2018}, doi = {10.1101/274456}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Multiplexed IF imaging using same species antibodies is in great demand as multiplexed IF is a general practice in biological and clinical laboratories whilst antibodies from different species are hardly available. We here presented a robust method to eliminate the use of secondary antibodies by replacing secondary antibody signal amplification with DNA-based Hybridization-Chain-Reaction signal amplification. We achieved as much as 89-fold signal enhancement, and four target imaging using all mouse primary antibodies.}, URL = {https://www.biorxiv.org/content/early/2018/03/02/274456}, eprint = {https://www.biorxiv.org/content/early/2018/03/02/274456.full.pdf}, journal = {bioRxiv} }