TY - JOUR T1 - A stable mode of bookmarking by TBP recruits RNA Polymerase II to mitotic chromosomes JF - bioRxiv DO - 10.1101/257451 SP - 257451 AU - Sheila S. Teves AU - Luye An AU - Aarohi Bhargava-Shah AU - Liangqi Xie AU - Xavier Darzacq AU - Robert Tjian Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/03/02/257451.abstract N2 - How a cell maintains transcriptional fidelity across mitosis has remained an enduring mystery in biology. One challenge arises during mitosis when chromatin becomes condensed and transcription is shut off. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remains bound globally to active promoters in ES cells during mitosis. Using live-cell single-molecule imaging, we observed that TBP mitotic binding is highly stable, with an average residence time of minutes. This stable binding is in stark contrast to typical TFs with residence times of seconds. To test the functional effect of mitotic TBP binding, we used a drug-inducible degron system and found that TBP promotes the association of RNA Polymerase II with mitotic chromosomes, and facilitates transcriptional reactivation following mitosis. These results suggest that the core transcriptional machinery maintains global transcriptional memory during mitosis. ER -