RT Journal Article
SR Electronic
T1 Alternative splicing of circadian clock genes is temperature regulated in field-grown sugarcane
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 718890
DO 10.1101/718890
A1 Luíza L. B. Dantas
A1 Cristiane P. G. Calixto
A1 Maira M. Dourado
A1 Monalisa S. Carneiro
A1 John W. S. Brown
A1 Carlos T. Hotta
YR 2019
UL http://biorxiv.org/content/early/2019/07/30/718890.abstract
AB Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of intron-containing genes in Arabidopsis, 46% in rice and 38% in maize and it is widespread among the circadian clock genes. Little is known about how AS influences the circadian clock of C4 plants, like commercial sugarcane, a C4 crop with a complex hybrid genome. This work aims to identify AS events in core circadian clock genes and the dynamic adjustment of the events as a response to temperature changes in field-grown sugarcane. A systematic search for AS in five sugarcane clock genes, ScLHY, ScPRR37, ScPRR73, ScPRR95 and ScTOC1 using different organs of sugarcane sampled during winter, with 4 months old plants, and during summer, with 9 months old plants, revealed temperature- and organ-dependent expression of at least one alternatively spliced isoform in all genes. Expression of AS isoforms varied according to the season. Our results suggest that AS events in circadian clock genes are regulated by temperature.