PT  - JOURNAL ARTICLE
AU  - David Tapia
AU  - Agustín Barría
AU  - José M. Yáñez
TI  - Comparison of Mortality and Viral Load in Rainbow Trout (<em>Oncorhynchus mykiss</em>) infected with Infectious Pancreatic Necrosis Virus (IPNV) Genogroups 1 and 5
AID  - 10.1101/719104
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 719104
4099  - http://biorxiv.org/content/early/2019/07/30/719104.short
4100  - http://biorxiv.org/content/early/2019/07/30/719104.full
AB  - Infectious Pancreatic Necrosis Virus (IPNV) is the etiological agent of a highly contagious disease that affects farmed salmonids. IPNV isolates have been phylogenetically classified into eight genogroups, of which two are present in Chile, genogroups 1 and 5. Here we compare the mortality rate caused by isolates from both genogroups in rainbow trout (Oncorhynchus mykiss) fry to determine if there is an association between host susceptibility and phylogenetic characterization of IPNV. Fish were challenged by immersion with one of four isolates (two of each genogroup) and mortality curves were assessed after 30 days. Viral load was measured in all mortalities and in live fish sampled at 1, 7 and 20 days post-infection. Although mortality was low throughout the challenge, differences were found between fish infected with different isolates. Both isolates from genogroup 1, caused greater cumulative mortalities than either of the isolates from Genogroup 5. When combined, the overall mortality rate of fish challenged with genogroup 1 isolates was significantly higher than those infected with genogroup 5. However, viral load was lower on trout infected with genogroup 1 isolates. These results suggest that rainbow trout are more susceptible to IPNV isolates from genogroup 1 than genogroup 5.