RT Journal Article
SR Electronic
T1 miR-21-5p and miR-30a-5p are identical in human and bovine, have similar isomiR distribution, and cannot be used to identify xenomiR uptake from cow milk
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 275834
DO 10.1101/275834
A1 Bastian Fromm
A1 Juan Pablo Tosar
A1 Lin Yu
A1 Marc K. Halushka
A1 Kenneth W. Witwer
YR 2018
UL http://biorxiv.org/content/early/2018/03/05/275834.abstract
AB microRNAs (miRNAs) are often highly conserved across species, but species-specific sequences are known. In addition, miRNA “isomiRs” arise from the same precursor molecule but differ in post-processing length and modification, usually at the 3’ end. A recently published feeding study reported the intriguing result that two bovine milk-specific miRNAs were taken up into human circulation after ingestion of bovine milk. Unfortunately, this interpretation is based on annotation errors in a public microRNA database. Reanalysis using databses including the MirGeneDB database reveals that the miRNAs in question, miR-21-5p and miR-30a-5p, arise from 100% identical 5’ precursor sequences in human and bovine, and the putative bovine-specific isomiRs appear to be depleted, not enriched, in bovine milk. Thus, enrichment of these isomiRs in human blood is inconsistent with uptake of xenomiRs and likely betrays endogenous miRNA regulation in response to diet or technical artifact.