PT  - JOURNAL ARTICLE
AU  - Caroline Hoffmann
AU  - Floriane Noel
AU  - Maximilien Grandclaudon
AU  - Paula Michea
AU  - Aurore Surun
AU  - Lilith Faucheux
AU  - Philemon Sirven
AU  - Olivier Lantz
AU  - Juliette Rochefort
AU  - Jerzy Klijanienko
AU  - Charlotte Lecerf
AU  - Maud Kamal
AU  - Christophe Le Tourneau
AU  - Maude Guillot-Delost
AU  - Vassili Soumelis
TI  - Pdl1 and icosl discriminate human secretory and helper dendritic cells
AID  - 10.1101/721563
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 721563
4099  - http://biorxiv.org/content/early/2019/08/01/721563.short
4100  - http://biorxiv.org/content/early/2019/08/01/721563.full
AB  - Dendritic cells (DC) are described as immature at the steady state, with a high antigen capture capacity, turning into a mature state with a strong T cell stimulatory capacity upon activation. Using 16 different stimuli in vitro (130 observations), we describe two states of human activated dendritic cells. PDL1highICOSLlow “secretory DC” produced large amounts of inflammatory cytokines and chemokines but induced very low levels of T helper (Th) cytokines following DC-T co-culture; conversely PDL1lowICOSLhigh “helper DC” produced low levels of secreted factors but induced high levels of Th cytokines characteristic of a broad range of Th subsets. Secretory DC were phenotypically identified in T cell inflamed primary head and neck squamous cell carcinoma. RNAseq analysis showed that they expressed a typical secretory DC signature, including CD40, PVR, IL1B, TNF, and CCL19. This novel and universal functional dichotomy of human DC opens broad perspectives for the characterization of inflammatory diseases, and for immunotherapy.