RT Journal Article
SR Electronic
T1 Pdl1 and icosl discriminate human secretory and helper dendritic cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 721563
DO 10.1101/721563
A1 Caroline Hoffmann
A1 Floriane Noel
A1 Maximilien Grandclaudon
A1 Paula Michea
A1 Aurore Surun
A1 Lilith Faucheux
A1 Philemon Sirven
A1 Olivier Lantz
A1 Juliette Rochefort
A1 Jerzy Klijanienko
A1 Charlotte Lecerf
A1 Maud Kamal
A1 Christophe Le Tourneau
A1 Maude Guillot-Delost
A1 Vassili Soumelis
YR 2019
UL http://biorxiv.org/content/early/2019/08/01/721563.abstract
AB Dendritic cells (DC) are described as immature at the steady state, with a high antigen capture capacity, turning into a mature state with a strong T cell stimulatory capacity upon activation. Using 16 different stimuli in vitro (130 observations), we describe two states of human activated dendritic cells. PDL1highICOSLlow “secretory DC” produced large amounts of inflammatory cytokines and chemokines but induced very low levels of T helper (Th) cytokines following DC-T co-culture; conversely PDL1lowICOSLhigh “helper DC” produced low levels of secreted factors but induced high levels of Th cytokines characteristic of a broad range of Th subsets. Secretory DC were phenotypically identified in T cell inflamed primary head and neck squamous cell carcinoma. RNAseq analysis showed that they expressed a typical secretory DC signature, including CD40, PVR, IL1B, TNF, and CCL19. This novel and universal functional dichotomy of human DC opens broad perspectives for the characterization of inflammatory diseases, and for immunotherapy.