PT  - JOURNAL ARTICLE
AU  - Joseph O. Magliozzi
AU  - Jack Sears
AU  - Marielle Brady
AU  - Hannah E. Opalko
AU  - Arminja N. Kettenbach
AU  - James B. Moseley
TI  - Defining how Pak1 regulates cell polarity and cell division in fission yeast
AID  - 10.1101/722900
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 722900
4099  - http://biorxiv.org/content/early/2019/08/02/722900.short
4100  - http://biorxiv.org/content/early/2019/08/02/722900.full
AB  - Protein kinases direct polarized growth by regulating the cytoskeleton in time and space, and could play similar roles in cell division. We found that the Cdc42-activated polarity kinase Pak1 colocalizes with the assembling cytokinetic ring and remains at the division site during septation. Mutations in pak1 led to defects in cytokinetic ring assembly and cell separation. Through a phosphoproteomic screen, we identified novel Pak1 targets that function in polarized growth, cytokinesis, and septation. For cytokinesis, we found that Pak1 regulates the localization of its substrates Mid1 and Cdc15 to the cytokinetic ring. For cell separation, Pak1 phosphorylates the RNA-binding protein Sts5 to prevent its assembly into granules. These results show that Pak1 acts directly on components of the cytokinetic ring, and unexpectedly promotes the later stages of cell division by inhibiting the assembly of ribonucleoprotein granules. More broadly, our work reveals that cell polarity signaling proteins coordinate diverse events to promote cell division at the end of the cell cycle.SUMMARY Magliozzi et al. show that fission yeast Pak1 regulates multiple events during cell division. Through a phosphoproteomic screen and subsequent mutant analysis, their work uncovers direct targets and mechanisms for Pak1 activity during cell growth and division.