PT  - JOURNAL ARTICLE
AU  - Tae Yeon Yoo
AU  - Jeong-Mo Choi
AU  - Che-Hang Yu
AU  - Rohit V. Pappu
AU  - Daniel J. Needleman
TI  - Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments
AID  - 10.1101/276212
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 276212
4099  - http://biorxiv.org/content/early/2018/03/07/276212.short
4100  - http://biorxiv.org/content/early/2018/03/07/276212.full
AB  - Proper kinetochore-microtubule attachments, mediated by the NDC80 complex, are required for error-free chromosome segregation. Erroneous attachments are corrected by the tension dependence of kinetochore-microtubule interactions. It has been difficult to establish the molecular basis of this process because of the lack of techniques to quantify NDC80 binding in vivo. Here, we present a method, based on fluorescence lifetime imaging microscopy and Förster resonance energy transfer, to quantitatively measure the fraction of NDC80 complexes bound to microtubules at individual kinetochores in living human cells. We found that NDC80 binding is modulated in a chromosome autonomous fashion over prometaphase and metaphase, and is predominantly regulated by centromere tension. We show that the tension dependency of NDC80 binding requires the proper localization of Aurora B kinase, which modulates NDC80 binding. Our results lead to a mathematical model of the molecular basis of tension-dependent NDC80 binding to kinetochore microtubules in vivo.