RT Journal Article
SR Electronic
T1 Essential role of Bone morphogenetic protein 15 in porcine ovarian and follicular development and ovulation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 724096
DO 10.1101/724096
A1 Yufeng Qin
A1 Tao Tang
A1 Wei Li
A1 Zhiguo Liu
A1 Xiaoliang Yang
A1 Xuan Shi
A1 Guanjie Sun
A1 Xiaofeng Liu
A1 Min Wang
A1 Xinyu Liang
A1 Peiqing Cong
A1 Delin Mo
A1 Xiaohong Liu
A1 Yaosheng Chen
A1 Zuyong He
YR 2019
UL http://biorxiv.org/content/early/2019/08/02/724096.abstract
AB Bone morphogenetic protein 15 (BMP15) is a multifunctional oocyte-specific secreted factor. It controls female fertility and follicular development in both species-specific and dosage-sensitive manners. Previous studies found that BMP15 played a critical role on follicular development and ovulation rate of mono-ovulatory mammalian species, but has minimal impact on poly-ovulatory mice. However, whether this is true in non-rodent poly-ovulatory species need to be validated. To investigate this question, we generated a BMP15 knockdown pig model. We found that BMP15 knockdown gilts showed markedly reduced fertility accompanied with phenotype of dysplastic ovaries containing significantly declined number of follicles, increased number of abnormal follicles, and abnormally enlarged antral follicles resulting in disordered ovulation. Molecular and transcriptome analysis revealed that knockdown of BMP15 significantly suppressed cell proliferation, differentiation, Fshr expression, leading to premature luteinization and reduced estradiol production in GCs, and simultaneously decreased the quality and meiotic maturation of oocyte. Our results provide in vivo evidences for the essential role of BMP15 in porcine ovarian and follicular development, and new insight into the complicated regulatory function of BMP15 in female fertility of poly-ovulatory species.