RT Journal Article
SR Electronic
T1 Structural diversity of oligomeric β-propellers with different numbers of identical blades
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 723619
DO 10.1101/723619
A1 Evgenia Afanasieva
A1 Indronil Chaudhuri
A1 Jörg Martin
A1 Eva Hertle
A1 Astrid Ursinus
A1 Vikram Alva
A1 Marcus D. Hartmann
A1 Andrei N. Lupas
YR 2019
UL http://biorxiv.org/content/early/2019/08/02/723619.abstract
AB β-Propellers arise through the amplification of a supersecondary structure element called a blade. This process produces toroids of between four and twelve repeats, which are almost always arranged sequentially in a single polypeptide chain. We found that new propellers evolve continuously by amplification from single blades. We therefore investigated whether such nascent propellers can fold as homo-oligomers before they have been fully amplified within a single chain. One-to six-bladed building blocks derived from two seven-bladed WD40 propellers yielded stable homo-oligomers with six to nine blades, depending on the size of the building block. High-resolution structures for tetramers of two blades, trimers of three blades, and dimers of four and five blades, respectively, show structurally diverse propellers and include a novel fold, highlighting the inherent flexibility of the WD40 blade. Our data support the hypothesis that subdomain-sized fragments can provide structural versatility in the evolution of new proteins.