RT Journal Article
SR Electronic
T1 The TRPC6-AMPK pathway is involved in cytoskeleton reorganization and glucose uptake in podocytes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 278911
DO 10.1101/278911
A1 Patrycja Rachubik
A1 Maria Szrejder
A1 Dorota Rogacka
A1 Irena Audzeyenka
A1 Michał Rychłowski
A1 Stefan Angielski
A1 Agnieszka Piwkowska
YR 2018
UL http://biorxiv.org/content/early/2018/03/08/278911.abstract
AB Podocytes are dynamic polarized cells on the surface of glomerular capillaries that are an essential part of the glomerular filtration barrier. AMP-activated protein kinase (AMPK), a key regulator of glucose and fatty acid metabolism, plays a major role in obesity and type 2 diabetes. Accumulating evidence suggests that TRPC6 channels are crucial mediators of calcium transport in podocytes and are involved in regulating glomerular filtration barrier. Here we investigated whether the AMPK-TRPC6 pathway is involved in insulin-dependent cytoskeleton reorganization and glucose uptake in cultured rat podocytes. Insulin regulates the interaction of TRPC6 with AMPKα2 in cultured rat podocytes The results suggested a key role for the TRPC6 channel in the mediation of insulin-dependent activation of AMPKα2, actin cytoskeleton reorganization and glucose uptake in podocyte. Moreover, AMPK and TRPC6 activation were required to stimulate the Rac1 signaling pathway. These results suggest a potentially important new mechanism that regulates glucose transport in podocytes and that could be injurious during diabetes.