RT Journal Article
SR Electronic
T1 GUN1 influences the accumulation of NEP-dependent transcripts and chloroplast protein import in Arabidopsis cotyledons upon perturbation of chloroplast protein homeostasis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 724518
DO 10.1101/724518
A1 Luca Tadini
A1 Carlotta Peracchio
A1 Andrea Trotta
A1 Monica Colombo
A1 Ilaria Mancini
A1 Nicolaj Jeran
A1 Alex Costa
A1 Franco Faoro
A1 Milena Marsoni
A1 Candida Vannini
A1 Eva-Mari Aro
A1 Paolo Pesaresi
YR 2019
UL http://biorxiv.org/content/early/2019/08/05/724518.abstract
AB Correct chloroplast development and function require coordinated expression of chloroplast and nuclear genes. This is achieved through chloroplast signals that modulate nuclear gene expression in accordance with the chloroplast’s needs. Genetic evidence indicates that GUN1, a chloroplast-localized pentatricopeptide-repeat (PPR) protein with a C-terminal Small MutS-Related (SMR) domain, is involved in integrating multiple developmental and stress-related signals in both young seedlings and adult leaves. Recently, GUN1 was found to interact physically with factors involved in chloroplast protein homeostasis, and with enzymes of tetrapyrrole biosynthesis in adult leaves that function in various retrograde signaling pathways. Here we show that, following perturbation of chloroplast protein homeostasis i) by growth in lincomycin-containing medium, or ii) in mutants defective in either the FtsH protease complex (ftsh), plastid ribosome activity (prps21-1 and prpl11-1) or plastid protein import and folding (cphsp70-1), GUN1 influences NEP-dependent transcript accumulation during cotyledon greening and also intervenes in chloroplast protein import.