RT Journal Article SR Electronic T1 De novo genome assembly of the meadow brown butterfly, Maniola jurtina JF bioRxiv FD Cold Spring Harbor Laboratory SP 715243 DO 10.1101/715243 A1 Kumar Saurabh Singh A1 David J. Hosken A1 Nina Wedell A1 Richard ffrench-Constant A1 Chris Bass A1 Simon Baxter A1 Konrad Paszkiewicz A1 Manmohan D Sharma YR 2019 UL http://biorxiv.org/content/early/2019/08/05/715243.abstract AB Background Meadow brown butterflies (Maniola jurtina) on the Isles of Scilly represent an ideal model in which to dissect the links between genotype, phenotype and long-term patterns of selection in the wild - a largely unfulfilled but fundamental aim of modern biology. To meet this aim, a clear description of genotype is required.Findings Here we present the draft genome sequence of M. jurtina to serve as an initial genetic resource for this species. Seven libraries were constructed using DNA from multiple wild caught females and sequenced using Illumina, PacBio RSII and MinION technology. A novel hybrid assembly approach was employed to generate a final assembly with an N50 of 214 kb (longest scaffold 2.9 Mb). The genome encodes a total of 36,294 genes. 90.3% and 88.7% of core BUSCO (Benchmarking Universal Single-Copy Orthologs) Arthropoda and Insecta gene sets were recovered as complete single-copies from this assembly. Comparisons with 17 other Lepidopteran species placed 86.5% of the assembled genes in orthogroups.Conclusions Our results provide the first high-quality draft genome and annotation of the butterfly M. jurtina.