TY - JOUR T1 - A streamlined method for transposon mutagenesis of <em>Rickettsia parkeri</em> yields numerous mutations that impact infection JF - bioRxiv DO - 10.1101/277160 SP - 277160 AU - Rebecca L. Lamason AU - Natasha M. Kafai AU - Matthew D. Welch Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/03/08/277160.abstract N2 - The rickettsiae are obligate intracellular alphaproteobacteria that exhibit a complex infectious life cycle in both arthropod and mammalian hosts. As obligate intracellular bacteria, Rickettsia are highly adapted to living inside a variety of host cells, including vascular endothelial cells during mammalian infection. Although it is assumed that the rickettsiae produce numerous virulence factors that usurp or disrupt various host cell pathways, they have been challenging to genetically manipulate to identify the key bacterial factors that contribute to infection. Motivated to overcome this challenge, we sought to expand the repertoire of available rickettsial loss-of-function mutants, using an improved mariner-based transposon mutagenesis scheme. Here, we present the isolation of over 100 transposon mutants in the spotted fever group species Rickettsia parkeri. These mutants targeted genes implicated in a variety of pathways, including bacterial replication and metabolism, hypothetical proteins, the type IV secretion system, as well as factors with previously established roles in host cell interactions and pathogenesis. Given the need to identify critical virulence factors, forward genetic screens such as this will provide an excellent platform to more directly investigate rickettsial biology and pathogenesis. ER -