RT Journal Article
SR Electronic
T1 Accurate measures of translation efficiency and traffic using ribosome profiling
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 719302
DO 10.1101/719302
A1 Juraj Szavits-Nossan
A1 Luca Ciandrini
YR 2019
UL http://biorxiv.org/content/early/2019/08/06/719302.abstract
AB Ribosome profiles are often interpreted with underlying naive models that are unable to grasp the complexity of the biological process. We develop a Non-Equilibrium Analysis of Ribo-seq (NEAR), and provide unprecedented estimates of translation initiation and elongation efficiencies, emphasising the importance of rating these two stages of translation separately. When examining ribosome profiling in yeast, we observe that translation initiation and elongation are close to their optima, and traffic is minimised at the beginning of the transcript to favour ribosome recruitment. However, we find many sites of congestion along the mRNAs where the probability of ribosome interference can reach 50%. Our analysis excludes unreliable datapoints and discusses biases of the experimental technique; as a result, we conclude that current analyses and definitions of translation efficiency are not adapt to quantitatively asses ribosome interference.