TY - JOUR T1 - KCC2 overexpression prevents the paradoxical seizure-promoting action of somatic inhibition JF - bioRxiv DO - 10.1101/279539 SP - 279539 AU - Vincent Magloire AU - Jonathan Cornford AU - Andreas Lieb AU - Dimitri M. Kullmann AU - Ivan Pavlov Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/03/09/279539.abstract N2 - Although cortical interneurons are apparently well-placed to suppress seizures, several recent reports have highlighted a paradoxical role of parvalbumin-positive perisomatic-targeting (PV+) interneurons in ictogenesis. Here, we use an acute in vivo model of focal cortical seizures in awake behaving mice, together with closed-loop optogenetic manipulation of PV+ interneurons, to investigate their function during seizures. We show that photo-depolarization of PV+ interneurons rapidly switches from an anti-ictal to a pro-ictal effect within a few seconds of seizure initiation. The pro-ictal effect of delayed photostimulation of PV+ interneurons was not shared with dendrite-targeting somatostatin-positive (SOM+) interneurons. We also show that this switch can be prevented by overexpression of the neuronal potassium-chloride co-transporter KCC2 in principal cortical neurons. These results suggest that strategies aimed at improving the ability of principal neurons to maintain intracellular chloride levels in the face of excessive network activity can prevent interneurons from contributing to seizure perpetuation. ER -