RT Journal Article
SR Electronic
T1 CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 280495
DO 10.1101/280495
A1 Baohui Chen
A1 Wei Zou
A1 Bo Huang
YR 2018
UL http://biorxiv.org/content/early/2018/03/12/280495.abstract
AB A lack of efficient tools to image non-repetitive genes in living cells has limited our ability to explore the functional impact of spatiotemporal dynamics of genes. Here, we addressed this issue by developing the CRISPR-Tag system as a new DNA tagging strategy to label protein-coding genes with high signal-to-noise ratio under wild-field fluorescence microscopy by using 1 to 4 highly active sgRNAs. The CRISPR-Tag, with minimal size of ∼ 250 bp, represents an easily and broadly applicable technique to study spatiotemporal organization of genomic elements in living cells.