PT  - JOURNAL ARTICLE
AU  - Karen J. Meaburn
AU  - Tom Misteli
TI  - Assessment of the Utility of Gene Positioning Biomarkers in the Stratification of Prostate Cancers
AID  - 10.1101/728972
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 728972
4099  - http://biorxiv.org/content/early/2019/08/08/728972.short
4100  - http://biorxiv.org/content/early/2019/08/08/728972.full
AB  - In eukaryotic cells, the genome is spatially organized in a non-random fashion within the confines of the interphase nucleus, and most genes occupy preferred nuclear positions. For some genomic loci these positioning patterns are context specific, reflected in the distinct location of certain genes and chromosomes in different cell types and in disease. Disease-related differential spatial positioning of genes has led to the hypothesis that the spatial reorganization of the genome may be utilized as a diagnostic biomarker. In keeping with this possibility, the positioning patterns of specific genes can be used to reproducibly discriminate benign tissues from cancerous ones. In addition to the use of spatial genome organization for diagnostic purposes, we explore here the potential use of spatial genome organization as a prognostic tool. This is a pressing need since in many cancer types there is a lack of accurate markers to predict the aggressiveness of individual tumors. We find that directional repositioning of SP100 and TGFB3 gene loci stratifies prostate cancers of differing Gleason scores. A more peripheral position of SP100 and TGFB3 in the nucleus, compared to benign tissues, is associated with low Gleason score cancers, whereas more internal positioning correlates with higher Gleason scores. Conversely, LMNA is more internally positioned in many non-metastatic prostate cancers, while its position is indistinguishable from benign tissue in metastatic cancer. Our findings of subtype-specific gene positioning patterns in prostate cancer provides a proof-of-concept for the potential usefulness of spatial gene positioning as a prognostic biomarker.BACbacterial artificial chromosomeEDTEuclidean distance transformFISHfluorescence in situ hybridizationFFPEformalin-fixed paraffin embeddedGPBgene positioning biomarkerHSAhuman chromosomeKS testKolmogorov-Smirnov testNATnormal adjacent to tumorPNDpooled normal distributionPSAserum prostate specific antigenRRDrelative radial distributionTMAtissue microarray